Publications and patents

RESAB and THERMCHEM project

  1. Pellegrini, V.; Lei, N.; Kyasaram, M.; Olsen, J. P.; Badino, S.; Windahl, M. S.; Colussi, F.; Cruys-Bagger, N.; Borch, K.; Westh, P.: Reversibility of substrate adsorption for the cellulases Cel7A, Cel6A and Cel7B from H. jecorina. Langmuir (Accepted - in press) 2014.
  2. Kari, J.; Olsen, J. P.; Borch, K.; Jensen, K.; Westh, P.: Kinetics of cellobiohydrolase (Cel7A) variants with lowered substrate affinity. Journal of Biological Chemistry (Accepted - in Press) 2014.
  3. Cellobiohydrolase variants and polynucleotides encoding same WO 2014138672 A1
  4. Cellobiohydrolase variants and polynucleotides encoding same WO 2014064115 A1
  5. Olsen, S. N.; Borch, K.; Cruys-Bagger, N.; Westh, P.: The role of product inhibition as a yield-determining factor in enzymatic high-solid hydrolysis of pretreated corn stover. App. Biochem. Biotech. 2014, 174, 146-155.
  6. Cruys-Bagger, N.; Tatsumi, H.; Borch, K.; Westh, P.: A graphene screen-printed carbon electrode for real-time measurements of unoccupied active sites in a cellulase. Anal. Biochem. 2014, 447, 162-168.
  7. Cruys-Bagger, N.; Badino, S. F.; Tokin, R.; Gontsarik, M.; Fathalinejad, S.; Jensen, K.; Toscano, M. D.; Sorensen, T. H.; Borch, K.; Tatsumi, H.; Valjamae, P.; Westh, P.: A pyranose dehydrogenase-based biosensor for kinetic analysis of enzymatic hydrolysis of cellulose by cellulases. Enzyme and Microbial Technology 2014, 58-59, 68-74.
  8. Alasepp, K.; Borch, K.; Cruys-Bagger, N.; Badino, S.; Jensen, K.; Sorensen, T. H.; Windahl, M. S.; Westh, P.: In Situ Stability of Substrate-Associated Cellulases Studied by DSC. Langmuir 2014, 30, 7134-7142.
  9. Murphy, L.; Bohlin, C.; Baumann, M. J.; Olsen, S. N.; Sørensen, T. H.; Andersson, L.; Borch, K.; Westh, P.: Product inhibition of five Hypocrea jecorina cellulases. Enzyme and Microbial Technology 2013, 52, 163-169.
  10. Cruys-Bagger, N.; Tatsumi, H.; Ren, G. R.; Borch, K.; Westh, P.: Transient Kinetics and Rate-Limiting Steps for the Processive Cellobiohydrolase Cel7A: Effects of Substrate Structure and Carbohydrate Binding Domain. Biochemistry 2013, 52, 8938-8948.
  11. Cruys-Bagger, N.; Elmerdahl, J.; Praestgaard, E.; Borch, K.; Westh, P.: A steady-state theory for processive cellulases. Febs J. 2013, 280, 3952-3961.
  12. Bohlin, C.; Praestgaard, E.; Baumann, M. J.; Borch, K.; Praestgaard, J.; Monrad, R. N.; Westh, P.: A comparative study of hydrolysis and transglycosylation activities of fungal beta-glucosidases. Appl. Microbiol. Biotechnol. (In Press) 2013, 97, 159-169.
  13. Murphy, L.; Cruys-Bagger, N.; Damgaard, H. D.; Baumann, M. J.; Olsen, S. N.; Borch, K.; Lassen, S. F.; Sweeney, M.; Tatsumi, H.; Westh, P.: Origin of Initial Burst in Activity for Trichoderma reesei endo-Glucanases Hydrolyzing Insoluble Cellulose. J. Biol. Chem. 2012, 287, 1252-1260.
  14. Cruys-Bagger, N.; Ren, G.; Tatsumi, H.; Baumann, M. J.; Spodsberg, N.; Andersen, H. D.; Gorton, L.; Borch, K.; Westh, P.: An amperometric enzyme biosensor for real-time measurements of cellobiohydrolase activity on insoluble cellulose. Biotechnology and Bioengineering 2012, 109, 3199-3204.
  15. Cruys-Bagger, N.; Elmerdahl, J.; Praestgaard, E.; Tatsumi, H.; Spodsberg, N.; Borch, K.; Westh, P.: Pre-steady-state Kinetics for Hydrolysis of Insoluble Cellulose by Cellobiohydrolase Cel7A. J. Biol. Chem. 2012, 287, 18451-18458.
  16. Praestgaard, E.; Elmerdahl, J.; Murphy, L.; Nymand, S.; McFarland, K. C.; Borch, K.; Westh, P.: A kinetic model for the burst phase of processive cellulases. FEBS J. 2011, 278, 1547-1560.
  17. Olsen, S. N.; Lumby, E.; McFarland, K.; Borch, K.; Westh, P.: Kinetics of Enzymatic High-Solid Hydrolysis of Lignocellulosic Biomass Studied by Calorimetry. Appl. Biochem. Biotechnol. 2011, 163, 626-635
  18. Olsen, S. N.; Bohlin, C.; Murphy, L.; Borch, K.; McFarland, K.; Sweeney, M.; Westh, P.: Effects of non-ionic surfactants on the interactions between cellulases and tannic acid: A model system for cellulase–poly-phenol interactions. Enz. Microb. Technol. 2011, 49, 353-359.
  19. Baumann, M. J.; Murphy, L.; Lei, N.; Krogh, K. B. R. M.; Borch, K.; Westh, P.: Advantages of isothermal titration calorimetry for xylanase kinetics in comparison to chemical reducing end assays. Anal. Biochem. 2011, 410, 19-26.
  20. Baumann, M. J.; Borch, K.; Westh, P.: Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity. Biotechnol. Biofuels 2011, 4, 45.
  21. Sotoft, L. F.; Westh, P.; Christensen, K. V.; Norddahl, B.: Novel investigation of enzymatic biodiesel reaction by isothermal calorimetry. Thermochim. Acta 2010, 501, 84-90.
  22. Murphy, L.; Borch, K.; McFarland, K. C.; Bohlin, C.; Westh, P.: A Calorimetric Assay For Enzymatic Saccharification Of Biomass. Enz. Microb. Technol. 2010, 46 141–146.
  23. Murphy, L.; Baumann, M. J.; Borch, K.; Sweeney, M.; Westh, P.: An enzymatic signal amplification system for calorimetric studies of cellobiohydrolases. Anal. Biochem. 2010, 404, 140-148.
  24. Bohlin, C.; Olsen, S. N.; Morant, M. D.; Patkar, S.; Borch, K.; Westh, P.: A Comparative Study of Activity and Apparent Inhibition of Fungal beta-Glucosidases. Biotechnol. Bioeng. 2010, 107, 943-952.